萱草花瓣中类胡萝卜素分析样品的制备及UPCC-MS检测方法研究

以大苞萱草（Hemerocallis middendorfii Trautv.et Mey.）和‘原谅’萱草（H.‘Pardon Me’）为研究对象，对萱草属植物花瓣中类胡萝卜素的样品制备方法以及UPCC-MS定性和定量检测方法进行了研究。结果表明：（1）萱草花瓣类胡萝卜素样品制备过程中，不同的提取试剂、振荡方法及皂化方法对类胡萝卜素的提取效率均有显著影响，经过对提取结果的方差分析，确定最佳的样品制备方案为：提取试剂B丙酮：正己烷（3：5/V：V）、温控摇床振荡提取，常温皂化16 h；（2） UPCC-MS技术能在10 min内高效分离萱草花瓣中的类胡萝卜素，且使用的有毒化学试剂少，是检测类胡萝卜素的较好选择；（3）大苞萱草和原谅萱草花瓣中共含有20种类胡萝卜素物质，两者颜色不同，类胡萝卜素的组成和含量也存在差异。

Sample Preparation and UPCC-MS Determination Method of Carotenoids in Petals of Hemerocallis

The petals of Hemerocallis middendorfii Trautv. et Mey. and H. ‘Pardon Me’ were used to study the sample preparation method of carotenoids, and the samples were qualitatively and quantitatively analyzed by UPCC-MS. Results showed that:(1)In the process of carotenoids sample preparation, different extraction solvents, shaking methods and saponification methods have significant influence on the extraction efficiency of carotenoids, and by multi-factor analysis of variance, the optimal extraction method was determined as follows:B acetone:hexane(3:5/V:V) as extraction solvents, extracted in a shaking incubator and saponification 16 h at room temperature; (2)UPCC-MS technology can separate the carotenoids in samples efficiently in 10 min, and with lesser use of toxic chemicals, UPCC-MS can considered to be a good choice for analyzing carotenoids; (3)There are 20 kinds of carotenoids in H.middendorfii and H. ‘Pardon Me’ petals, and these two kinds of materials with different colors had different composition and content of carotenoids.