Excretion of benzo[a]pyrene-Gua adduct in the urine of benzo[a]pyrene-treated rats |
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| Authors: | H Autrup T Seremet |
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| Affiliation: | 1. School of Chemistry and Chemical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China;2. Hirano Institute for Materials Innovation, Shanghai Jiao Tong University, Shanghai 200240, China;1. Center for Esophageal Diseases and Swallowing, Division of Gastroenterology and Hepatology, Department of Medicine, University of North Carolina, Chapel Hill, NC, United States;2. Department of Genetics, University of Chapel Hill, NC, United States;3. Miraca Life Sciences Research Institute, Irving, TX, United States;4. Dallas Veterans Affairs Medical Center, University of Texas Southwestern Medical Center, Dallas, TX, United States |
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| Abstract: | A benzo[a]pyrene(BP)-Gua adduct was extracted in the urine of rats treated with BP. Some (0.15%) of the administered dose of BP was excreted as BP-Gua within 48 h. A double labelling experiment demonstrated that the excreted product contained both a BP and a Gua moiety. Partially hepatectomized rats treated with [14C]Gua during the regenerative phase were injected with [3H]BP and the urine collected and processed by chromatographic procedures. The adduct had similar chromatographic properties to the adduct released from human PLC/5 cells treated with 7,8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE) and co-chromatographed with 7-BPDE-Gua released from BPDE-adducted DNA under aqueous conditions. Detection and quantitation of BP-Gua offers an alternative, non-invasive method of monitoring individuals exposed to carcinogenic polycyclic aromatic hydrocarbons (PAHs). |
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