| 脱血红素细胞色素c自发折叠的进一步验证 |
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| 引用本文： | 韩学海,杨福愉. 脱血红素细胞色素c自发折叠的进一步验证[J]. 中国生物化学与分子生物学报, 1996, 12(5): 593-598 |
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| 作者姓名： | 韩学海 杨福愉 |
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| 作者单位： | 中国科学院生物物理研究所生物大分子国家重点实验室 |
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| 基金项目： | 国家自然科学基金,中国科学院资助 |
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| 摘 要： | 用胰蛋白酶水解结合聚丙烯酰胺凝胶电泳以及纳秒内源荧光衰减谱分析对鸡心脱血红素细胞色素ｃ在透析复性过程的自发折叠现象作进一步确定。结果显示随着透析复性时间的增加，鸡心脱血红素细胞色素ｃ对胰蛋白酶的水解敏感性逐渐下降，内源荧光寿命值增大，与此对应的马心脱血红素细胞色素ｃ没有发生变化。
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| 关 键 词： | 脱血红素细胞色素c 蛋白质折叠 胰蛋白酶水解 敏感性 |
| 收稿时间： | 1996-10-20 |
Further Study on the Spontaneous Partial Folding of Chicken Heart Apocytochrome c |
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| Han Xue-Hai,Yang Fu-Yu. Further Study on the Spontaneous Partial Folding of Chicken Heart Apocytochrome c[J]. Chinese Journal of Biochemistry and Molecular Biology, 1996, 12(5): 593-598 |
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| Authors: | Han Xue-Hai Yang Fu-Yu |
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| Affiliation: | (National Laboratory of Biomacromolecules, Institute of BiophysicsIcademia Sinica, Beijing 100101 |
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| Abstract: | The spontaneous folding characteristics of chicken heart apocytochrome c was further examined by trypsin hydrolysis followed by SDS-PAGE and analysis of the intrinsic fluorescence decay spectra. The results showed that ,in contrast to horse heart apocytochrome c, hydrolysis accessibility of chicken heart apocytochrome c to trypsin was decreasing while the fluorescence lifetime was increasing with increasing in dialysis time. It was suggested that chicken heart apocytochrome c concomitant with the spontaneous partial folding tends to aggregate and its N-terminal may not involve in the spontaneous. folding. |
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| Keywords: | Apocytochrome c Spontaneous folding Trypsin hydrolysis Accessibility |
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