PCR detection of the lactocin S structural gene in bacteriocin-producing lactobacilli from meat. |
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Authors: | J M Rodrí guez, L M Cintas, P Casaus, A Su rez, P E Hern ndez |
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Affiliation: | J M Rodríguez, L M Cintas, P Casaus, A Suárez, and P E Hernández |
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Abstract: | The lactocin S structural gene (lasA) in seven bacteriocinogenic lactobacilli isolated from fermented sausages was studied. Two degenerate primers were synthesized to amplify a 75-bp fragment of the gene. Three strains amplified the fragment from their plasmid DNA, and hybridization analysis confirmed these results. |
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