| 烟草悬浮细胞在凋亡过程中Ca2+分布的共聚焦显微成像研究 |
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| 引用本文： | 王蒴,于维军,林英,孙忠思.烟草悬浮细胞在凋亡过程中Ca2+分布的共聚焦显微成像研究[J].激光生物学报,2011,20(1):9-14. |
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| 作者姓名： | 王蒴 于维军 林英 孙忠思 |
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| 作者单位： | 1. 沈阳农业大学分析测试中心,辽宁,沈阳,110866
2. 沈阳农业大学生物技术学院,辽宁,沈阳,110866 |
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| 基金项目： | 沈阳农业大学青年科研基金 |
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| 摘 要： | 以Fluo-3AM为游离Ca2+荧光探针,利用激光扫描共聚焦显微镜（Laser Scanning Confocal Microscope,LSCM）对烟草悬浮细胞在热激诱导的凋亡过程中Ca2+时空分布进行了研究。结果显示,在正常细胞中,Ca2+集中分布在细胞壁和细胞核中,细胞质中分布较少;在凋亡早期细胞中,细胞质中Ca2+的浓度增加;在凋亡晚期的细胞中,细胞核中的Ca2+浓度增加较为明显。结果提示,在凋亡过程中,Ca2+的定位分布存在一定的规律。
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| 关 键 词： | 烟草悬浮细胞 凋亡 Ca2+ Fluo-3 激光扫描共聚焦显微镜 |
A Study on the Distribution of Ca2+ in the Process of Apoptosis in Tobacco Suspension Cells by Laser Scanning Confocal Microscope |
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| WANG Shuo,YU Wei-jun,LIN Ying,SUN Zhong-si.A Study on the Distribution of Ca2+ in the Process of Apoptosis in Tobacco Suspension Cells by Laser Scanning Confocal Microscope[J].ACTA Laser Biology Sinica,2011,20(1):9-14. |
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| Authors: | WANG Shuo YU Wei-jun LIN Ying SUN Zhong-si |
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| Institution: | WANG Shuo~(a*),YU Wei-jun~a,LIN Ying~b,SUN Zhong-si~b (Shenyang Agricultural University a.Center for Instrumental Analysis,b.Biological Science and Technology College,Shenyang 110866,Liaoning,China) |
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| Abstract: | The tobacco suspension cells in apoptosis induced by heat shock were loaded with Fluo-3AM,which was a free Ca~(2+) fluorescent indicator.The time-space distribution of Ca~(2+) were measured by LSCM(Laser Scanning Confocal Microscope) in the process of apoptosis.The results showed,in the control group,the concentration of Ca~(2+) in the cytoplasm were lower than that in the cell wall and the cell nucleus;in the early phase,the concentration of Ca~(2+) in the cytoplasm increased;in the later phase,the free Ca... |
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| Keywords: | Ca2+ Fluo-3 |
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