Effects of complex extracellular matrices on 5-azacytidine-induced myogenesis |
| |
| Authors: | Timothy Scott-Burden Emil Bogenmann Peter A Jones |
| |
| Institution: | 1. Department of Medical Biochemistry, University of Cape Town Medical School, Observatory, 7925, South Africa;2. Division of Hematology and Oncology, Childrens Hospital of Los Angeles, Los Angeles, CA 90027, USA;3. Urological Cancer Research Laboratory, USC Cancer Center, Los Angeles, CA 90033, USA;1. College of Pharmaceutical Sciences, Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Key Laboratory of Marine Fishery Resources Exploitment, Zhejiang Province, China;2. Zhejiang University, School Medicine, Affiliated Hospital 2, Department of Surgery, Hangzhou 310009, China;1. Departamento de Diagnóstico en Patología y Medicina Oral, Facultad de Odontología, Universidad de la República, Uruguay;2. Departamento de Genómica, Instituto de Investigaciones Biológicas Clemente Estable, Uruguay;3. Instituto de Física, Facultad de Ingeniería, Universidad de la República, Montevideo, Uruguay;4. Sección Biología Celular, Facultad de Ciencias, Universidad de la República, Uruguay;5. Departamento de Odontología Conservadora, Faculdade de Odontología, Universidade Federal do Río Grande Do Sul, Brazil;6. Departamento de Biología Odontológica, Facultad de Odontología, Universidad de la República, Uruguay;1. Institute of Biomedical Engineering and Nanomedicine, National Health Research Institutes, Miaoli County, Taiwan;2. Department of Materials Science and Engineering, National United University, Miaoli County, Taiwan;3. Department of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan;4. PhD Program in Tissue Engineering and Regenerative Medicine, National Chung Hsing University, Taichung, Taiwan;5. Institute of Biotechnology and Pharmaceutical Research, National Health Research Institutes, Miaoli County, Taiwan;6. Taipei Cancer Center, Taipei Medical University Hospital, Taipei, Taiwan;7. TMU Research Center of Cancer Translational Medicine, Taipei Medical University, Taipei, Taiwan |
| |
| Abstract: | Culture dishes coated with extracellular matrix material synthesized by bovine endothelial cells, rat smooth muscle cells or human fibroblasts were used to study proliferation and myogenesis in C3H/10T1/2 C18 (10T1/2) cells primed to differentiate with 5-azacytidine (5-aza-CR). Endothelial and smooth muscle matrices were permissive for growth and myogenic differentiation of treated 10T1/2 cells, whereas the fibroblast matrix was inhibitory. All three types of matrix-coated dishes were refractory for myogenesis after brief exposure to trypsin. Analysis of the matrix glycosaminoglycans showed that high chondroitin sulfate relative to hyaluronic acid (HA) levels were favorable for the myogenic response. The ratio between these two glycosaminoglycans therefore had a major influence on mesenchymal differentiation. These results using complex extracellular matrices produced in vitro may be useful in understanding cell-matrix interactions during embryogenesis. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
