水稻10kDa富硫醇溶蛋白基因家族中一个假基因拷贝的核苷酸序列分析

本研究以中花１０号水稻为材料，利用ＰＣＲ技术扩增并克隆１０ｋＤａ富硫醇溶蛋白基因的成熟肽编码区。对扩增产物的核苷酸序列分析表明：本扩增产物长３８０ｂｐ；与Ｍａｓｕｍｕｒａ等人［１］发表的序列相比，有９４．５％的同源性，与我们以前发表的中花１０号水稻１０ｋＤａ富硫醇溶蛋白基因的序列相比，有９９．５％的同源性。本扩增片段第１５０位与１５１位间的单碱基（Ｔ）插入导致了该片段编码区的移码突变，并在其后的第１６２至１６４位处形成了一琥珀终止子（ＴＡＧ）。这表明水稻１０ｋＤａ富硫酸蛋白基因家族中确实存在不正常的假基因拷贝。

Sequence analysis of a pseudogeue member in the 10kDa sulfur-rich prolamin gene family or the Chinese rice

In this paper, the 10kDa sulfur-rich prolamin gene was amplified by PCR, cloned and sequenced from Chinese rice. The sequence analysis showed that the amplified fragment was 380hp long, 94. 5% homologous to the nucleic acid sequence reported by Masumura et al. 1] and 99. 5% homologous to our previous re sultsl2]. The single base insertion between position 150 and 151 caused the reading frame mutation of the fragment and formed an amber codon at position between 162 to 164. These results showed that there would be a pseudogene member in the 10kDa sulfur-rich prolamin gene family.