Inductive interactions required for mesodermal differentiation in Bufo arenarum gastrulae |
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Authors: | M. E. Manes Fernando Campos Casal |
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Affiliation: | (1) Cátedra de Histología y Embriología, Facultad de Agronomía y Zootecnia, Universidad Nacional de Tucumán, 4000, Tucumán, Argentina, AR |
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Abstract: | Dorso-marginal epithelium, a distinct crescent-shaped region in the early gastrula of Bufo arenarum, appears after involution as a narrow dorso-median strip of archenteric endoderm close to the notochord. In explant cultures, this layer showed an extreme dorsalizing activity, promoting the formation of notochordal structures from ventro-mesodermal cells. In the presence of ectoderm, this inductive activity was expanded resulting in a wide range of dorso-lateral components such as notochord, muscle, nephric tubules, mesothelium and mesenchyme. The mesodermal origin of these derivatives was confirmed by the use of FDA (fluorescein-dextran-amine)-labelled explants. Extensive mesodermal development in cultures seems to require cell contacts between the inner aspect of the dorso-marginal epithelium and mesodermal cells. When such contacts were prevented, cultures would only differentiate erythrocytes as a result of a purely ectodermal stimulus. Bisection of the dorso-marginal epithelium in whole embryos resulted in the development of a duplicated set of axial structures, clearly showing the role of the epithelium as a dorsal organizer. Received: 30 July 1996 / Accepted: 20 February 1997 |
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Keywords: | amphibian morphogenesis organizer mesoderm dorsalization differentiation |
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