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A New Method of Synthesis of Fluorescently Labelled Oligonucleotides and Their Application in DNA Sequencing |
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| Authors: | Wojciech T. Markiewicz Gabriele Gröger Rudi Rösch Anna Zebrowska Hartmut Seliger |
| Affiliation: | 1. Institute of Bioorganic Chemistry, Polish Academy of Sciences , Noskowskiego 12/14, PL-61704, Poznan, Poland;2. Universit?t Ulm , Sektion Polymere, Oberer Eselsberg, D-7900, Ulm, Germany |
| Abstract: | Abstract A new approach to the chemical synthesis of oligonucleotides bearing reporter functional groups based on the modification of cytosine residues during the final deprotection step is reported. The application of the fluorescently labelled primer for the automated DNA sequencing is shown. |
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