Synthesis of Modified Oligonucleotides and Production of Duplexes With Covalently Linked Chains |
| |
| Authors: | S. I. Antsypovich T. S. Oretskaya E. M. Volkov E. A. Romanova V. N. Tashlitsky M. Blumenfeld |
| |
| Affiliation: | 1. Chemical Department , Moscow State University , Moscow, 119899, Russia;2. GENSET, 1 Rue R. et S. Delaunay , Paris, 75011, France |
| |
| Abstract: | Abstract A method has been devised to synthesize a DNA-duplex with covalently connected strands. Primary amino group located on one strand is linked to a carboxyl group of the other strand through the agency of a water soluble carbodiimide condensing agent. Conditions for the reaction between chains of a duplex composed of the modified oligonucleotides [1] were optimized. The thermal and hydrolytic stability of the cross linked duplex was examined. |
| |
| Keywords: | |
|
|
