An Improved Method for the Preparation of Methyl dichlorophosphite. A Key Reagent In the Phosphite Method of Oligonucleotide Synthesis |
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| Authors: | Z Tashma I Ringel J Deutsch S Cohen M Weisz J Katzhendler |
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| Institution: | 1. Department of Pharmaceutical Chemistry , The Hebrew University School of Pharmacy , Jerusalem, Israel;2. Department of Pharmacology , The Hebrew University Hadassah Medical School , Jerusalem, Israel |
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| Abstract: | Abstract The reaction between phosphorus trichloride (PCl3) and trimethyl phosphite (CH3O)3p, has been examined by 31Pnmr, in order to achieve a simple and efficient procedure for the formation of methyl dichlorophosphite (CH3OPCl2), which is a key intermediate in the synthesis of oligonucleotides. The yield of the reaction was also studied on a preparative scale and it was found that the optimal condition is obtained when the reactants molar ratio is 1:1. |
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