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Enhanced Fluorescence in the Binding of Oligonucleotides With a Pyrene Group in the Sugar Fragment to Complementary Polynucleotides |
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| Authors: | Kazushige Yamana Tadao Gokota Hiroaki Ozaki Hidehiko Nakano Osamu Sangen Takeo Shimidzu |
| Affiliation: | 1. Department of Applied Chemistry , Himeji Institute of Technology , 2167 Shosha, Himeji, 671-22, Japan;2. Division of Molecular Engineering , Graduate School of Engineering, Kyoto University , Sakyo-ku, Kyoto, 606, Japan |
| Abstract: | Abstract The fluorescence intensity and lifetime of oligonucleotides with a pyrenylmethyl group at the specific sugar residue were increased upon binding to their complementary polynucleotide in aqueous solution. The present oligonucleotide-pyrene conjugates provide new fluorescent probes for detection of specific nucleic acids. |
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