Site-Specific 13C Labeling of DNA to Deduce DNA Repair Mechanisms of Uracil-DNA Glycosyiase and UV Endonuclease V |
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| Authors: | M. Manoharan S. C. Ransom A. Mazumder J. A. Gerlt |
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| Affiliation: | 1. Leukemia Society of America Research Fellow 1986-89;2. Department of Chemistry Biochemistry , University of Maryland , College Park, Maryland, 20742, USA;3. Lifecodes Corporation , Valhalla, NY, 10595 |
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| Abstract: | Abstract The oligodeoxynucleotide d(GCGUGCG) was synthesized with [1′,3′ -13C2)U labeling. The uracil unit was removed with uracil-DNA glycosylase to generate an abasic site and the resulting oligonucleotide was paired with the possible d(CGCNCGC) structures. One of these heteroduplexes was a substrate for W endonuclease V. The 13C NMR spectra of these heteroduplexes describe the structure of the abasic site and the mechanism of the endonuclease reaction. |
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