Improved Impurity Profile of Phosphorothioate Oligonucleotides Through the Use of Dimeric Phosphoramidite Synthons |
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Authors: | Achim H. Krotz Patrick Klopchin Douglas L. Cole Vasulinga T. Ravikumar |
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Affiliation: | Isis Pharmaceuticals , 2292 Faraday Avenue, Carlsbad, CA, 92008, USA Fax: E-mail: VRAVIKUMAR@ISISPH.COM |
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Abstract: | Abstract Phosphorothioate oligonucleotides synthesized through assembly of dimeric phosphoramidite synthons show a significantly improved impurity profile compared to oligomers synthesized through coupling of standard monomer phosphoramidites. A greater than 70% reduction of the (n-1)-mer population and a ca. 50% reduction of phosphodiester linkages has been achieved. |
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