Delivery of Polynucleotides with Polyamine Lipids and Polymers |
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| Authors: | B A Demeneix O Boussif M A Zanta J S Remy J P Behr |
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| Institution: | 1. Laboratoire de Physiologie Générale et Comparée, U.R.A. 90 , Museum National d'Histoire Naturelle , 75231, Paris Cedex;2. Laboratoire de Chimie Génétique, URA 1386, Faculté de Pharmacie , Université Louis Pasteur de Strasbourg , 67401, Illkirch, France |
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| Abstract: | Abstract Several non-permanent polycations possessing substantial buffering capacity below physiological pH, such as lipopolyamines and polyethylenimines, are efficient transfection agents per se, i.e. without the addition of lysosomotropic bases, or cell targeting or membrane disruption agents. These vectors have been shown to deliver genes as well as oligonucleotides both in vitro and in vivo. Our hypothesis is that their efficiency relies on extensive endosome swelling and rupture that provides an escape mechanism for the polycation/DNA particles. |
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