Enzymic Phosphorylation of Some 5-Aminoimidazole Nucleosides to the 5′-Phosphates |
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| Authors: | Robert W Humble Grahame Mackenzie Gordon Shaw |
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| Institution: | 1. School of Science, Humberside College of Higher Education , Hull, HU6 7RT;2. School of Chemistry, University of Bradford , Bradford, W. Yorkshire, BD7 1DP, (U.K.) |
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| Abstract: | Abstract Phosphotransferases from wheat shoots and a suitable phosphate donor have been used for the specific conversion of unprotected pyrimidine and purine nucleosides and some analogues into nucleoside 5'-phosphates. We have been interested to investigate the application of this technique to the phosphorylation of 5-aminoimidazole nucleosides to afford corresponding 5'-phosphates related to intermediates in purine nucleotide de novo biosynthesis. Several D-ribofuranosyl, D-xylofuranosyl and D-arabinofuranosyl 5-aminoimidazoles have been successfully phosphorylated (TABLE) to 5'-phosphates using a phosphotransferase from wheat shoots and p-nitrophenylphosphate as a phosphate donor. |
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