Fast Rna-Rna Annealing in Vitro: Single Cycle Selection Versus Multiple Cycle Selection |
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| Authors: | Volker Patzel Ralf Kronenwett Karola Rittner Georg Sczakiel |
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| Institution: | 1. Deutsches Krebsforschungszentrum , Im Neuenheimer Feld 242, D-69120, Heidelberg , Germany;2. TRANSGENE S.A. , 11 rue de Molsheim, 67000, Strasbourg , France. |
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| Abstract: | Abstract Fast annealing of complementary RNA in vitro is related to effective antisense RNA-mediated regulation of gene expression and inhibition of viral replication in living cells. Pools of antisense RNA can be selected for species that anneal fast with a given target strand. In this study, we compare the technical and biological advantages and disadvantages of a single round selection assay with extensive multiple cycle selection for fast-annealing antisense RNA species. |
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