Effects of Psoralen-Derivatized Oligonucleoside Methylphosphonates on Vesicular Stomatitis Virus (VSV) Protein Synthesis In Vitro and in VSV-Infected Cells |
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| Authors: | Joel T Levis Joanne M Kean Paul S Miller |
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| Institution: | Department of Biochemistry , School of Hygiene and Public Health, The Johns Hopkins University , 615 North Wolfe Street, Baltimore , Maryland , 21205 |
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| Abstract: | Abstract Oligodeoxyribonucleoside methylphosphonates (16-mers) targeted to VSV mRNAs were derivatized with 4′-{N-(aminoethyl)amino]methyl}-4,5′8-trimethylpsoralen. These oligomers specifically inhibit translation of their targeted mRNAs in vitro following UV irradiation of the oligomer-mRNA complexes. Psoralen-derivatized oligonucleoside methylphosphonates are stable in cells and can inhibit VSV protein synthesis in culture following UV-irradiation of VSV-infected cells. |
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