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Design and Chemoenzymatic Synthesis of A Tissue Plasminogen Activator Gene with Unique Restriction Sites: A Model for Studying Protein Domain Function |
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| Authors: | N. Theriault Hatzenbuhler E. F. Rehberg J. B. Carter D. P. Palermo E. V. Hubert P. W. Bergum |
| Affiliation: | 1. Biopolmer Chemistry, The Upjohn Company , Kalamazoo, MI, 49001;2. Molecular Biology, The Upjohn Company , Kalamazoo, MI, 49001;3. Metabolic Diseases Research, The Upjohn Company , Kalamazoo, MI, 49001 |
| Abstract: | Abstract We have designed and constructed a cassette tPA gene with unique restriction sites in the interdomain regions. Each domain was chemoenzymatically assembled, cloned and sequenced. We have constructed and expressed the full-length molecule and some deletion mutants. |
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