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Semi-Mechanized Simultaneous Synthesis of Multiple Oligonucleotide Fragments |
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| Authors: | H. Seliger A. Herold U. Kotschi J. Lyons G. Schmidt F. Eisenbeiß |
| Affiliation: | 1. Univ. Ulm , Sektion Polymere, Oberer Eselsberg, D 7900, Ulm, F.R. Germany;2. E. Merck , Zentrallaboratorium Reagenzienforschung, Frankfurter Str. 250, D 6100, Darmstadt, F.R. Germany |
| Abstract: | Abstract A new strategy for the simultaneous synthesis of multiple oligonucleotides is described, which is based on asynchronous chain growth and use of a maximum number of support segments in each elongation. An approach to the mechanization of this strategy and applications to the preparation of gene fragments and sequentially variant probes are discussed. |
| Keywords: | 2‐Amino‐6‐substituted‐purine nucleosides and 2′‐deoxynucleosides 2′‐Deoxyguanosine derivatives Guanosine derivatives Guanine nucleoside modifications |