Structural Determination of Silicon-Containing Oligonucleotides by 1H−29Si Long-Range Heteronuclear Multiple Quantum Correlation NMR Spectroscopy |
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| Authors: | James M Hewitt William C Lenhart Richard N Moore Ashis K Saha Alexander L Weis |
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| Institution: | 1. Eastman Kodak Company , 1999 Lake Avenue, Rochester , NY , 14650-2132;2. Eastman Kodak Company , 1999 Lake Avenue, Rochester , NY , 14650-2132;3. Chemagnetics , Fort Collins , CO.;4. Sterling Research Group, Sterling Drug Inc. , 25 Great Valley Parkway, Malvern , PA , 19355 |
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| Abstract: | Abstract The technique of 1H?29Si Long-Range Heteronuclear Multiple Quantum Correlation NMR Spectroscopy was used to determine the structure of silicon-containing oligonucleotides. Trimers which contained silicon instead of phosphorus as part of the oligonucleotide link were synthesized through a synthetic route that required minimal hydroxyl protection. The resulting trimer could have one of two possible structures. Through the use of 1H?29Si HMQC NMR spectroscopy, it was possible to link the 3′-hydroxymethine proton of one sugar to the 5′-hydroxymethylene proton of an adjacent sugar by correlation to the same silicon atom, thus elucidating the final structure. |
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