Enhanced Specificity of Minimally Modified Anti-c-myc Oligonucleotides |
| |
| Authors: | A Peyman H Ragg T Ulshöfer D W Will E Uhlmann |
| |
| Institution: | Hoechst Marion Roussel, Pharma Research , D-65926, Frankfurt, Germany |
| |
| Abstract: | Abstract The sequence-specificity of antisense oligonucleotides (ODN) against c-myc mRNA was tested by Northern blot analysis. Rat smooth muscle cells were treated with antisense or control ODN against c-myc modified by the “minimal protection strategy”. At 0.3 μM concentration the ODN show a very specific reduction in c-myc mRNA levels. Use of the “minimal protection strategy” minimizes nonspecific effects as observed for all-phosphorothioate ODN containing four consecutive guanine residues. |
| |
| Keywords: | |
|
|
