Properties of Oligonucleotides Containing the Transition Bases P and K |
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| Authors: | F Hill D Loakes D M Brown |
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| Institution: | Medical Research Council, Laboratory of Molecular Biology , Hills Road, Cambridge, CB2 2QH, UK |
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| Abstract: | Abstract The properties of the degenerate nucleosides dP and dK, in templates and primers were determined. dP was copied as either pyrimidine, dK as either purine. In primers, an equimolar mixture of the two nucleosides functioned as a universal base equivalent in both sequencing and the polymerase chain reactions. Cloning of DNA containing dP or dK produced transition mutations in vivo. |
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