Differentiation of reptilian neural crest cells in Vitro |
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Authors: | Ling Hou Takuji Takeuchi |
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Institution: | (1) Biological Institute, Faculty of Science, Tohoku University, Aoba-yama, 980 Sendai, Japan |
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Abstract: | An attempt was made to culture neural crest cells of the turtle embryo in vitro. Trunk neural tubes from the St. 9/10 embryos
were explanted in culture dishes. The developmental potency of the turtle neural crest cells in vitro was shown to be essentially
similar to that of avian neural crest cells, although they seem to be more sensitive to melanocyte-stimulating hormone (MSH)
stimulation. We describe conditions under which explanted neural tube gives rise to neural crest cells that differentiate
into neuronal cells and melanocytes. The potency of melanocyte differentiation was, found to vary according to the concentration
of fetal bovine serum (FBS, from 5 to 20%). Melanization of neural crest cells cultured in the medium containing FBS and α-MSH
was more extensive than those cultured with FBS alone, combinations of FBS and chick embryo extract, or turtle embryo extract.
These culture conditions seem to be useful for the study of the developmental potency of the neural crest cells as well as
for investigating local environmental factors. |
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Keywords: | neural crest cells differentiation cell culture melanocytes α -melanocyte-stimulating hormone reptiles |
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