Characterization of the sugar-binding specificity of the toxic lectins isolated from Abrus pulchellus seeds |
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Authors: | Marcio V Ramos Alexandre H Sampaio Benildo S Cavada Juan J Calvete Thalles B Grangeiro Henri Debray |
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Institution: | (1) Departamentos de Biologia, Universidade Federal do Ceara, Campus do Pici, Cx. Postal 6033, Fortaleza-Ceara, Brasil, 60.451-970;(2) Engenharia de pesca, Universidade Federal do Ceara, Campus do Pici, Cx. Postal 6033, Fortaleza-Ceara, Brasil, 60.451-970;(3) Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Campus do Pici, Cx. Postal 6033, Fortaleza-Ceara, Brasil, 60.451-970;(4) Intituto de Biomedicina de Valencia, C.S.I.C., 1146010 Valencia, Spain;(5) Departamentos de Biologia, Brasil;(6) Laboratoire de Biochimie et UMR N.8576 du CNRS, Université des Sciences et Technologies de Lille, 59655 Villeneuve D'Ascq CEDEX, France |
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Abstract: | The sugar-binding specificity of the toxic lectins from Abrus pulchellus seeds was investigated by combination of affinity chromatography of glycopeptides and oligosaccharides of well-defined structures on a lectin-Sepharose column and measurement of the kinetic interactions in real time towards immobilized glycoproteins. The lectins showed strong affinity for a series of bi- and triantennary N-acetyllactosamine type glycans. The related asialo-oligosaccharides interact more strongly with the lectins. The best recognized structures were asialo-glycopeptides from fetuin. Accordingly, the kinetic interaction with immobilized asialofetuin was by far the most pronounced. Human and bovine lactotransferrins and human serotransferrin interacted to a lesser extent. The interaction with asialofetuin was inhibited by galactose in a dose dependent manner. Lactose, N-acetyllactosamine and lacto-N-biose exhibited similar degree of inhibition while N-acetylgalactosamine was a poor inhibitor. These results suggested that the carbohydrate-binding site of the Abrus pulchellus lectins was specific for galactose and possess a remarkable affinity for the sequences lactose -D-Gal-(1 4)-D-Glc], N-acetyllactosamine -D-Gal-(1 4)-D-GlcNAc] and lacto-N-biose -D-Gal-(1 3)-D-GlcNAc]. |
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Keywords: | Abrus pulchellus lectin plant toxin protein-carbohydrate interaction surface plasmon resonance |
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