A self-adapting approach for the detection of bursts and network bursts in neuronal cultures |
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Authors: | Valentina Pasquale Sergio Martinoia Michela Chiappalone |
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Affiliation: | 1. Neuroscience and Brain Technologies Department, Italian Institute of Technology, Via Morego 30, 16163, Genova, Italy 2. Neuroengineering and Bio-nanoTechnology Laboratory, Department of Biophysical and Electronic Engineering, University of Genova, Via all’Opera Pia 11A, 16145, Genova, Italy
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Abstract: | Dissociated networks of neurons typically exhibit bursting behavior, whose features are strongly influenced by the age of the culture, by chemical/electrical stimulation or by environmental conditions. To help the experimenter in identifying the changes possibly induced by specific protocols, we developed a self-adapting method for detecting both bursts and network bursts from electrophysiological activity recorded by means of micro-electrode arrays. The algorithm is based on the computation of the logarithmic inter-spike interval histogram and automatically detects the best threshold to distinguish between inter- and intra-burst inter-spike intervals for each recording channel of the array. An analogous procedure is followed for the detection of network bursts, looking for sequences of closely spaced single-channel bursts. We tested our algorithm on recordings of spontaneous as well as chemically stimulated activity, comparing its performance to other methods available in the literature. |
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