Antisense effect of pyrrolidine-based oxy-peptide nucleic acids in Escherichia coli |
| |
| Authors: | Kitamatsu Mizuki Kurami Shunsuke Ohtsuki Takashi Sisido Masahiko |
| |
| Institution: | Department of Medical and Bioengineering, Graduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushimanaka, Kita-ku, Okayama 700-0082, Japan |
| |
| Abstract: | To investigate the antisense effect of a pyrrolidine-based oxy-peptide nucleic acid (POPNA), we carried out the LacZ reporter assay using a 12-mer trans-l-POPNA conjugated with a cell-penetrating peptide (antisense reagent). The antisense effect of the conjugated POPNA (inhibition of LacZ activity) was comparable to that shown by a Nielsen-type peptide nucleic acid. Furthermore, the conjugated POPNA could switch the LacZ activity over a wide range of ambient temperatures. |
| |
| Keywords: | Peptide nucleic acid Cell-penetrating peptide Antisense effect |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
