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Phenylalanine Ammonia-Lyase Activity and Anthocyanin Accumulation in Wounded Maize Mesocotyls
Authors:S F Pascholati    R L Nicholson  L G Butler
Institution:Instituto Biologico, CP7119 Sao Paulo (Brazil).;Instituto Biologico, CP7119 Sao Paulo (Brazil).;Department of Biochemistry, Purdue University, W. Lafayette, Indiana 47907 (U.S.A.).
Abstract:Activity of phenylalanine ammonia-lyase (E.C. 4.3.1.5) and anthocyanin accumulation were determined in wounded maize (Zea mays L.) mesocotyls. Mesocotyls were wounded with aluminum oxide and were placed in a 15 h light: 9 h dark photoperiod or in the dark. Extractable enzyme activity increased in response to wounding in the photoperiod but not in the dark. Anthocyanin accumulation in mesocotyls placed in the photoperiod decreased in response to wounding. The results are discussed with reference to phenylalanine ammonia-lyase activity in mesocotyl tissue wounded or inoculated with fungal pathogens.
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