The role of calcium-permeable AMPA receptors in disynaptic feedforward inhibition in the rat prefrontal cortex |
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Authors: | A V Zaitsev K Kh Kim L G Magazanik |
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Institution: | 1.Sechenov Institute of Evolutionary Physiology and Biochemistry,Russian Academy of Sciences,St.Petersburg,Russia |
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Abstract: | Calcium-permeable AMPA receptors (CP-AMPARs) play an important role in synaptic transmission and plasticity, but they also
can induce neuronal death under certain pathological conditions. The involvement of CP-AMPARs in the pathogenesis of many
diseases of the central nervous system makes them an attractive target for selective pharmacological blockade, to prevent
and relieve pathological processes. However, the practical application of selective CP-AMPAR channel blockers requires a thorough
study of their effects on the functioning of neural networks under the normal conditions. The goal of this study was to clarify
the role of CP-AMPARs in the regulation of firing thresholds in different types of cortical neurons, as well as their involvement
in maintaining the excitation/inhibition balance in the cortex. To do this, we have investigated the effects of CP-AMPARs
blockade on the amplitude of excitatory postsynaptic potentials (EPSPs) and the threshold of action potentials evoked by extracellular
stimulation. Whole-cell current-clamp recordings were carried out from pyramidal cells and fast-spiking interneurons in the
slices of rat medial prefrontal cortex. CP-AMPARs were blocked with a selective channel blocker IEM-1460 (100 μM), the dicationic
derivative of adamantane. It was found that the blockade of CP-AMPARs reduced the amplitude EPSPs in interneurons but not
in pyramidal cells. In addition, it reduced the firing threshold in pyramidal cells via partial suppression of feedforward
inhibition. Thus, the blockade of CP-AMPA receptors shifts the balance between cortical excitation and inhibition toward excitation. |
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