In situ synthesis of fluorescent membrane lipids (ceramides) using click chemistry |
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| Authors: | María Garrido José Luis Abad Alicia Alonso Félix M. Goñi Antonio Delgado L.-Ruth Montes |
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| Affiliation: | 1.Consejo Superior de Investigaciones Científicas (CSIC), Research Unit on Bioactive Molecules (RUBAM),Institut de Química Avan?ada de Catalunya (IQAC-CSIC),Barcelona,Spain;2.Unidad de Biofísica (CSIC-UPV/EHU), and Departamento de Bioquímica,Universidad del País Vasco,Bilbao,Spain;3.Facultad de Farmacia, Unidad de Química Farmacéutica (Unidad Asociada al CSIC),Universidad de Barcelona (UB),Barcelona,Spain |
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| Abstract: | Ceramide analogues containing azide groups either in the polar head or in the hydrocarbon chains are non-fluorescent. When incorporated into phospholipid bilayers, they can react in situ with a non-fluorescent 1,8-naphthalimide using click chemistry giving rise to fluorescent ceramide derivatives emitting at ≈440 nm. When incorporated into giant unilamellar vesicles, two-photon excitation at 760 nm allows visualization of the ceramide-containing bilayers. This kind of method may be of general applicability in the study of model and cell membranes. |
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| Keywords: | Ceramide GUV Fluorescence Click chemistry |
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