The role of the carboxy-terminal membrane-spanning fragment in the biogenesis of Escherichia coli K12 outer membrane protein PhoE |
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Authors: | Dirk Bosch Monica Scholten Cora Verhagen Jan Tommassen |
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Affiliation: | (1) Department of Molecular Cell Biology and Institute for Molecular Biology, State University of Utrecht, Transitorium 3, Padualaan 8, NL-3584 CH Utrecht, The Netherlands |
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Abstract: | Summary PhoE protein of Escherichia coli K12 is an outer membrane protein which is supposed to span the membrane sixteen times. By creating a deletion which removes the last membrane-spanning fragment and studying the localization of the truncated PhoE, we show that this fragment is indispensable for trimerization and outer membrane localization. In addition, circumstantial evidence for the proposed topology model of the protein was obtained. An insertion mutation in a region supposed to be cell surface-exposed, interferes with the binding of a monoclonal antibody which recognizes a cell surface-exposed epitope of the protein. |
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Keywords: | PhoE porin Outer membrane protein Escherichia coli Biogenesis-topology |
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