A Simple Method for Isolating DNA of High Yield and Quality from Cotton (shape Gossypium hirsutum L.) Leaves |
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| Authors: | Hugo R. Permingeat Maria V. Romagnoli Juliana I. Sesma Ruben H. Vallejos |
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| Affiliation: | (1) Centro de Estudios Fotosinteticos y Bioquimicos, CEFOBI (CONICET, UNR, Fundacion Miguel Lillo), Suipacha 531 –, 2000 Rosario, Argentina |
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| Abstract: | An easy, reproducible and fast procedure to isolate DNA from cotton leaves is described. The addition of 0.5 M glucose in the extraction buffer avoids browning by polyphenolic compounds and improves the quality of DNA for molecular analysis. The DNA yield ranged between 150–400 mg per gram of fresh tissue. The DNA was suitable for digestion by restriction enzymes and amplificatiion by Taq DNA polymerase. |
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| Keywords: | cotton DNA extraction |
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