Enzymatic conversion of benzylpenicillin to 6-aminopenicillanic acid in concentrated ultrafiltered broths |
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Authors: | A M A Nabais J P Cardoso |
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Institution: | Laboratório de Engenharia Bioquímica, Instituto Superior Técnico, 1096 Lisboa Codex, Portugal, PT
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Abstract: | Benzylpenicillin filtered broths purified by ultrafiltration and fermented broths clarified by ultrafiltration and afterwards concentrated by reverse osmosis were used directly for enzymatic conversion of benzylpenicillin to 6-aminopenicillanic acid and phenylacetic acid by immobilised penicillin G acylase or amidase. It was concluded that, when the ultrafiltration operation retained 100% of protein, the concentrates from reverse osmosis could be successfully directly fed to the enzymatic reactor, giving high enzymatic conversion yield of benzylpenicillin to 6-aminopenicillanic acid. |
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