Characterization of a Drosophila centrosome protein CP309 that shares homology with Kendrin and CG-NAP |
| |
Authors: | Kawaguchi Shin-ichi Zheng Yixian |
| |
Affiliation: | Department of Embryology, Carnegie Institution of Washington and Howard Hughes Medical Institute, Baltimore, Maryland 21210, USA. |
| |
Abstract: | The centrosome in animal cells provides a major microtubule-nucleating site that regulates the microtubule cytoskeleton temporally and spatially throughout the cell cycle. We report the identification in Drosophila melanogaster of a large coiled-coil centrosome protein that can bind to calmodulin. Biochemical studies reveal that this novel Drosophila centrosome protein, centrosome protein of 309 kDa (CP309), cofractionates with the gamma-tubulin ring complex and the centrosome-complementing activity. We show that CP309 is required for microtubule nucleation mediated by centrosomes and that it interacts with the gamma-tubulin small complex. These findings suggest that the microtubule-nucleating activity of the centrosome requires the function of CP309. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|