Isoelectric focusing of human head hair keratins from various racial groups

Isoelectric focusing of extracted keratin not S-carboxymethylated followed by silver staining was used in this study with the attempt to identify head hair keratins from different racial groups: Guatuso and Caribbean (Costa Rica, America), Balanta (Guinea-Bissau, Africa) and Sardinian (Italy, Europe). Morphological analysis of hair and quantitation of solubilized total proteins were also performed. Keratins extracted from hair gave similar IEF patterns for all samples in the ranges of pH 4.5–5.0 and 5.4–7.4, differing only in the intensity and width of the bands. IEF patterns for Guatuso samples differ from all the others in the presence of some additional bands in the range of pH 4.0–4.5. Different electrophoretic patterns are not associated with detectable differences in the morphology of the fibers. It seems to us that electrophoretic techniques may be useful in the identification of hair from different racial groups.