Characterization of microbial communities of biofilters by phospholipid fatty acid analysis and rRNA targeted oligonucleotide probes |
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| Authors: | von Keitz V Schramm A Altendorf K Lipski A |
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| Institution: | 1. Research Centre for Natural Resources, Environment and Society (CERNAS), Polytechnic Institute of Coimbra, Coimbra, Escola Superior Agrária de Coimbra, Bencanta, 3045-601 Coimbra, Portugal;2. Environmental Management Center, Mykolas Romeris University, Ateities g. 20, LT-8303 Vilnius, Lithuania;3. Department of Physical Geography and Bolin Centre for Climate Research, Stockholm University, SE-106 91 Stockholm, Sweden;1. Dept. of Natural Sciences, Dickinson State University, Dickinson, ND, USA;2. AGES, Dept. for Soil Health and Plant Nutrition, Wien, Austria;3. CNR - Ibimet, Sesto Fiorentino (FI), Italy;4. Universidad de Sevilla, Facultad de Química, Dpto. de Cristalografía, Sevilla, Spain;5. Wroclaw University of Environmental and Life Sciences, Institute of Soil Science, Wroclaw Poland;6. Agronomy Department, Iowa State University, Ames, IA, USA;7. Leibniz-Center for Agricultural Landscape Research (ZALF) Müncheberg, Institute of Soil Landscape Research, Eberswalder Str. 84, 15374 Müncheberg, Germany;8. Environmental Management Center, Mykolas Romeris University, Ateities St., Vilnius, Lithuania;1. Department of Geography, University of Barcelona, Spain;2. Environmental Management Center, Mykolas Romeris University, Vilnius, Lithuania;3. Department of Géographie, Centre d''Études Nordiques, Université Laval, Canada;1. Centre for Geographical Studies — IGOT, Universidade de Lisboa, Alameda da Universidade, 1600-214 Lisbon, Portugal;2. Department for Physical and Regional Geography, University of Barcelona, Spain;3. Department for Regional and Physical Geography, Complutense University of Madrid, Spain;4. Department of Physics, University of Alcalá, Spain;5. Department of Forestry, Michigan State University, USA Environmental Management Center, Mykolas Romeris University, Lithuania;6. Department of Geography, University of Oviedo, Spain |
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| Abstract: | The microbial community of a biofilter for waste gas treatment of an animal rendering plant was characterized by the analyses of the phospholipid fatty acids (PLFAs) of the filter material. For these analyses five samples of one filter were taken in intervals between one and two months. The main components of the PLFA profiles were straight chain saturated, monounsaturated and cyclopropyl fatty acids. Terminally branched and 10-methyl branched fatty acids were present in minor amounts. The structure and succession of the microbial community was interpreted by the presence and quantitative changes of diagnostic fatty acids. The stability of diagnostic fatty acids in relation to varying incubation parameters was tested for a number of bacterial isolates from biofilters representing different phylogenetic branches. For two samples, the data from the PLFA-analyses were compared with data obtained by hybridization with fluorescently labeled, rRNA-targeted oligonucleotide probes specific for the alpha-, beta- and gamma-subclass of the Proteobacteria, the Actinobacteria (Firmicutes with high G+C content) and the Firmicutes with low G+C content. These data indicated a dominating number of Proteobacteria (54% and 35% of DAPI-stained cells), in which the gamma-Proteobacteria represented the main fraction. Actinobacteria were detected in minor amounts, the number of Firmicutes with low G+C content was near the detection limit of the method. About half of the cells detected with a probe specific for Bacteria did not hybridize with the probes specific for the alpha-, beta- and gamma subclass of the Proteobacteria and the two subgroups of the Firmicutes. The results of both methods, the fluorescence in situ hybridization (FISH) and the PLFA analyses corresponded well and were best suited to confirm and complement each other. |
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