| 细菌人工染色体（BAC）及其分析和修饰方法简介 |
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| 引用本文： | 黄粤,刘德培,梁植权. 细菌人工染色体（BAC）及其分析和修饰方法简介[J]. 生物化学与生物物理进展, 2001, 28(3): 309-313 |
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| 作者姓名： | 黄粤 刘德培 梁植权 |
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| 作者单位： | 中国医学科学院 |
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| 摘 要： | 细菌人工染色体是一种新发展起来的DNA载体系统，它具有容量大、遗传特性稳定、易于操作等优点.在基因文库构建和基因功能分析等方面有广泛的应用.综述了近年来发展起来的对BAC进行分析和修饰的一些方法.
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| 关 键 词： | 细菌人工染色体，定位修饰，同源重组 |
| 收稿时间： | 2000-06-22 |
| 修稿时间： | 2000-06-22 |
Advances in Analysis and Modification of Bacterial Artificial Chromosome |
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| HUANG Yue,LIU De-Pei and LIANG Zhi-Quan. Advances in Analysis and Modification of Bacterial Artificial Chromosome[J]. Progress In Biochemistry and Biophysics, 2001, 28(3): 309-313 |
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| Authors: | HUANG Yue LIU De-Pei LIANG Zhi-Quan |
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| Affiliation: | National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China;National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China;National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences,Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China |
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| Abstract: | As a new developmental vector system, the bacterial artificial chromosome (BAC) have several advantages: larger capacity, hereditary stability and easy handling, etc. It has been extensively used in construction of genomic library and analysis of gene function. Some new methods of analysis and modification of BAC were reviewed. |
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| Keywords: | bacterial artificial chromosome site-directed modification homologous recombination |
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