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Expression of NADH-Dependent Glutamate Synthase in Response to the Supply of Nitrogen in Rice Cells in Suspension Culture
Authors:Watanabe  Sachiko; Sakai  Takahiro; Goto  Satoshi; Yaginuma  Toshiko; Hayakawa  Toshihiko; Yamaya  Tomoyuki
Institution:Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, 981 Japan
Abstract:The effects of inorganic and organic nitrogen on the levelsof mRNA for NADH-dependent glutamate synthase (GOGAT) and theprotein were examined in rice cells in suspension culture. Asupply of NH+4, NO-3, glutamine, or asparagine induced the accumulationof the protein and mRNA, but levels of mRNA for ferredoxin-GOGATwere hardly affected. 1Present address: P.C. Center Wakuya-cho, Toda-gun, Miyagi,Japan.
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