鲤鱼体长性状的QTL定位及其遗传效应分析

以大头鲤/荷包红鲤抗寒品系的重组自交系群体及其遗传连锁图谱, 利用Windows Map Manager 2.0的标记回归法进行QTL单标记定位分析和复合区间作图法进行QTL区间检测, 通过置换实验(1 000次重复)确定连锁群显著性水平阈值。在体长性状的标记回归研究中, 共7个标记达到显著水平(P<0.01), 对性状的贡献率为14.00%～27.00%, 其中3个标记达到极显著水平(P<0.001)。HLJ534, HLJ319, HLJ370座位可能与影响鲤鱼体长性状的主效基因连锁。在体长性状的QTL区间定位研究中, 共6个QTL达到连锁群显著水平(P=0.05), 对性状的贡献率为11.33.%～23.12%, 其中2个达到连锁群极显著性水平(P=0.01), 它们的加性效应方向并不一致。HLJ190-HLJ497区间和HLJ479-HLJ483区间是影响鲤鱼体长性状的主效QTL区间。

Mapping and genetic effect analysis of quantitative trait loci re-lated to body size in common carp (Cyprinus carpio L.)

The common carp recombinant inbred lines (RIL) derived from the cross Barbless carp x Hebao-cold tolerance red carp were used as experimental materials in this study. Based on the linkage map constructed with simple sequence repeat (SSR) markers using this RIL population, marker regression and complexity interval mapping were analyzed by Windows Map Manager2.0 software. A P-value of 0.01 was the threshold value of single-marker. A linkage group-wide permutation test (1 000 replicates) determined the significance of the maximum LOD value over the various intervals analyzed for each linkage group. The results are as follows: 1) For maker regression, a total of 7 makers related body size, were at 1% significant level, of which 3 were at 0.1% significant level. The variance explained by these loci, ranged 14.00% to 27.00%. Loci HLJ534, HLJ319, and HLJ370 at 0.1% significant level closely linked to the gene related to body size of common carp; 2) six significant QTLs related to body size were at the 5% linkage group-wide level on these linkage groups, and two of them were at 1% level. The variances explained by these QTLs ranged from 11.33% to 23.12% and their additive effects were not identical. HLJ190-HLJ497 and HLJ479-HLJ483 were major QTLs associated to body size of common carp.