Optimizing recombinant protein expression in the T7 system under the control of the proUp promoter |
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Authors: | Yogender Pal Jagdish C Gupta KJ Mukherjee |
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Institution: | (1) Centre for Biotechnology, Jawaharlal Nehru University, New Delhi, India |
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Abstract: | -Galactosidase and streptokinase expression was tested under the control of the T7 promoter in batch and fed-batch cultures. An Escherichia coli host GJ1158, which contained the T7 RNA polymerase gene under the osmo-responsive proUp promoter, was used for expression studies. -Galactosidase expression was enhanced from 26 mg l–1 to 127 mg l–1 in batch culture when a combination of sucrose and sorbitol was used instead of salt as an inducer. Similarly in fed-batch cultures 140 mg streptokinase l–1 was formed with sucrose and sorbitol induction which was higher than that achieved with IPTG induced cultures. |
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Keywords: | -galactosidase" target="_blank">gif" alt="beta" align="MIDDLE" BORDER="0">-galactosidase proUp promoter streptokinase T7 promoter |
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