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Ley antigen expression is correlated with apoptosis (programmed cell death) |
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| Authors: | Hiraishi, Katsuya Suzuki, Keiji Hakomori, Sen-itiroh Adachi, Masakazu |
| Affiliation: | 1Japan Immunoresearch Laboratory Co., Ltd 351–1 Nishiyokote-cho, Takasaki-city, Gunma Prefecture 370 2Department of Pathology, College of Medical Care and Technology, Gunma University 3–39–22 Showa-cho, Maebashi-city, Gunma Prefecture 371, Japan, 3The Biomembrane Institute, 201 Elliott Avenue W, Seattle, WA 98119 and Departments of Pathobiology and Microbiology, University of Washington Seattle, WA 98195, USA |
| Abstract: | Apoptosis (programmed cell death) is a basic physiological processwhich determines specific patterns of tissue size and shape,and balance of cell number, during morphogenesis, and seemsto play an integral role in oncogenic progression. Since dramaticchanges of cellular glycosylation pattern are well known tobe closely correlated with differentiation, development andoncogenesis, it is likely that similar specific changes areassociated with apoptosis. However, this possibility has notbeen systematically investigated. We therefore carried out histologicalstudies of many tumours and normal tissues for which a highincidence of apoptosis is believed to occur. Sections were stainedwith monoclonal antibodies (MoAbs) directed to carbohydrateantigens Ley and Lex, proliferating cellular nuclear antigen(PCNA) and Fas (previously claimed to be an apoptosis-inducingantigen). Antibody staining patterns were compared with morphologicalcell characteristics as revealed by haematoxylin/eosin staining,and DNA fragmentation patterns (a marker of apoptosis) as revealedby 3'-OH nick-end labelling technique. We found that expressionof Ley (defined by MoAb BM1) is closely correlated with theprocess of apoptosis, but not with cell proliferation or necrosis.Within Ley-positive areas of tissue sections, typical apoptoticmorphological changes and DNA fragmentation (as revealed bypositive nick-end labelling) were frequently observed in certainloci, although not all Ley-positive cells showed such signsof apoptosis. Ley-positive areas showed consistent negativestaining by MoAb directed to PCNA and negative or weak stainingby MoAb directed to Fas antigen, regardless of tissue source.No such trends were observed for Lex glycosylation. We concludethat Ley expression is a useful phenotypic marker predictiveof apoptosis, i.e. some (although not all) Ley-positive cellssubsequently become apoptotic. apoptosis expression glycosylation patterns Ley antigen 3'-OH nick-end labelling |
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