A cDNA clone encoding Brassica calmodulin |
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| Authors: | Mee-Len Chye Chun-Ming Liu Chio-Tee Tan |
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| Affiliation: | (1) Institute of Molecular and Cell Biology, National University of Singapore, 0511 Singapore, Republic of Singapore;(2) Present address: Department of Botany, University of Hong Kong, Pokfulam Road, Hong Kong;(3) Department of Applied Genetics, John Innes Institute, NR4 7UH Norwich, UK |
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| Abstract: | A 834 bp cDNA encoding calmodulin (CaM) has been isolated from Brassica juncea. On Northern analysis this cDNA hybridises this cDNA to mRNAs of about 0.9 kb in leaf, silique and peduncle. Genomic Southern analysis indicates the presence of a CaM multigene family in Brassica juncea. Comparison of the predicted amino acid sequence of Brassica CaM with that of Arabidopsis CaM ACaM-2 and ACaM-3 showed 100% homology, which is not unusual, since both plants belong to the family Cruciferae. In situ hybridisation studies on Brassica seedlings using a digoxigenin-labelled RNA probe showed that high levels of CaM mRNA were detected in the leaf primordia and the shoot apical meristem, and to a lesser degree, in the zone of root elongation of the root tip. The occurrence of a higher rate of cell division and growth in these regions than its surrounding tissue may possibly be related to higher levels of CaM mRNA. |
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| Keywords: | calcium-binding protein Cruciferae in situ hybridisation shoot apical meristem root tip |
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