Physical characterization of the genome of the Myxococcus xanthus bacteriophage MX-8 |
| |
Authors: | Edmund Stellwag James M Fink and James Zissler |
| |
Institution: | (1) Department of Microbiology, University of Minnesota, 55455 Minneapolis, MN, USA |
| |
Abstract: | Summary We have constructed a restriction map for the genome of bacteriophage MX-8 from Myxococcus xanthus using the enzymes PvuII, MboI, and EcoRI. The phage genome size, as determined by restriction analysis, is 51.7±0.6 Kb. Double digestions, redigestions of isolated fragments, and crossed-contact hybridization of partial digestion products show that the restriction map is circular. Restriction analysis and Southern hybridization show that the phage DNA molecules are packaged sequentially from a concatemer starting from a specific site which we have mapped. The DNA molecules have an average terminal redundancy of approximately 8% and are circularly permuted over at least 40% of the genome. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|