Age-related changes in mature CD4+ T cells: cell cycle analysis |
| |
Authors: | Hale Timothy J Richardson Bruce C Sweet Leonard I McElligott David L Riggs James E Chu Elton B Glynn Jacqueline M LaFrenz Dave Ernst David N Rochford Rosemary Hobbs Monte V |
| |
Affiliation: | Department of Epidemiology, University of Michigan, Ann Arbor, MI 48109-0940, USA. |
| |
Abstract: | T cell proliferative responses decrease with age, but the mechanisms responsible are unknown. We examined the impact of age on memory and naive CD4(+) T cell entry and progression through the cell cycle using acridine orange to identify cell cycle stage. For both subsets, fewer stimulated cells from old donors were able to enter and progress through the first cell cycle, with an increased number of cells arrested in G(0) and fewer cells in post G(0) phases. The number of dead cells as assessed by sub-G(0) DNA was also significantly greater in the old group. CD4(+) T cells from old mice also exhibited a significant reduction in clonal history as assessed by CFSE staining. This was associated with a significant decline in cyclin D2 mRNA and protein. We propose that decreases in cyclin D2 are at least partially responsible for the proliferative decline found in aged CD4(+) T cells. |
| |
Keywords: | Rodent T Lymphocytes Cell surface molecules Cellular proliferation Costimulation Memory |
本文献已被 ScienceDirect PubMed 等数据库收录! |