Bioemulsifier production by a halothermophilic Bacillus strain with potential applications in microbially enhanced oil recovery |
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Authors: | S. M. M. Dastgheib M. A. Amoozegar E. Elahi S. Asad I. M. Banat |
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Affiliation: | (1) Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran;(2) Extremophiles Lab, Department of Microbiology, School of Biology, College of Science, University of Tehran, P.O. Box 14155-6455, Tehran, Iran;(3) Department of Cellular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran;(4) School of Biomedical Sciences, University of Ulster, Coleraine, BT52 1SA, Northern Ireland, UK |
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Abstract: | A halothermotolerant Gram-positive spore-forming bacterium was isolated from petroleum reservoirs in Iran and identified as Bacillus licheniformis sp. strain ACO1 by phenotypic characterization and 16S rRNA analysis. It showed a high capacity for bioemulsifier production and grew up to 60°C with NaCl at 180 g l−1. The optimum NaCl concentration, pH and temperature for bioemulsifier production were 4% (w/v), 8.0, and 45°C, respectively. Although ACO1 did not utilize hydrocarbons, it had a high emulsifying activity (E 24 = 65 ± 5%) on different hydrophobic substrates. Emulsification was optimal while growing on yeast extract as the sole carbon source and NaNO3 as the nitrogen source. The efficiency of the residual oil recovery increased by 22% after in situ growth of B. licheniformis ACO1 in a sand-pack model saturated with liquid paraffin. |
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Keywords: | Biosurfactant Emulsifier Enhanced oil recovery Sand-pack model Thermophile |
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