Density gradient analysis of newly replicated DNA from synchronized mouse lymphoma cells |
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Authors: | W G Flamm N J Bernheim P E Brubaker |
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Institution: | National Institute of Environmental Health Sciences, National Institutes of Health, Public Health Service, Department of Health, Education and Welfare, Research Triangle Park, N. C. 27709, USA |
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Abstract: | The replication of DNA in synchronous cultures of mouse lymphoma cells was investigated by use of CsCl density gradient centrifugation. We found that the buoyant density of newly replicated DNA depended upon the particular stage of S phase in which synthesis occurred. In early S phase, newly replicated DNA exhibited buoyant densities which were slightly higher, on the average, than that of pre-existing DNA. As S phase progressed, newly replicated DNA shifted to lower buoyant densities, until, near the end of S phase, densities less than pre-existing DNA were observed. These observations are discussed in terms of their possible relevance to base compositional differences between nucleotide sequences made in early as opposed to middle or late S phase. |
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