| 山羊草属异源多倍体植物基因组进化的ＲＡＰＤ分析 |
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| 引用本文： | 蔡从利,王建波,景润春,朱英国. 山羊草属异源多倍体植物基因组进化的ＲＡＰＤ分析[J]. 遗传学报, 2001, 28(2): 158-165 |
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| 作者姓名： | 蔡从利 王建波 景润春 朱英国 |
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| 作者单位： | 武汉大学生命科学学院, |
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| 基金项目： | 国家自然科学基金资助项目（39770056） |
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| 摘 要： | 和２４个随机引物对山羊草属（Ａegilops L.)异源多倍体物种对其祖先二倍体物进行ＲＡＰＤ分析，对扩增出的３１３条带进行聚类分析发现，含Ｄ基因组的多倍体与二倍体祖先Ａe.squarrosa(DD)在聚类图上聚为一支；除Ａe.juvenalis(DDMMUU)聚到上一支外，含Ｕ基因组的多倍 与二倍体祖先Ae.umbellulata(ＵＵ）在聚类图上聚为另一支；多倍体与其他二倍体均不聚在一起，表明多倍体分别与Ａe.squarrosa(DD)、Ae.umbellulata(UU)具有较近的亲缘关系，这说明多倍体开之后，Ｄ和Ｕ基因组变化较小，而其他基因组则发生了较大的变化。
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| 关 键 词： | 山羊草属 异源多倍体 基因组进化 RAPD 禾本科 小麦族 |
| 文章编号： | 0379-4172（2001）02-0158-08 |
| 修稿时间： | 2000-05-22 |
RAPD Analysis of theGenome Evolution in Allopolyploid Species in Aegilops |
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| CAI Cong-li,WANG Jian-Bo,JING Run-Chun,ZHU Ying-Guo. RAPD Analysis of theGenome Evolution in Allopolyploid Species in Aegilops[J]. Journal of Genetics and Genomics, 2001, 28(2): 158-165 |
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| Authors: | CAI Cong-li WANG Jian-Bo JING Run-Chun ZHU Ying-Guo |
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| Abstract: | RAPD analysis was performed by applying a set of 24 arbitrary rimers to all the allopolyploids and their diploid progenitors in Aegilops L. The analysis based on 313 RAPD fragments revealed that the allopolyploids carrying D genome grouped with Ae. squarrosa (DD) in one cluster. The allopolyploids carrying U genome grouped with Ae. umbellulata (UU) in another cluster except Ae. juvenalis (DDMMUU),which grouped with Ae. squarrosa (DD). The allopolyploids did not cluster with the other diploids. The results indicated that the allopolyploids shared close relationship with Ae. squarrosa (DD) and Ae. umbellulata (UU), respectively. It proved that the D and U genomes altered relatively little from the diploid progenitors after the allopolyploids came into being, but the other genomes changed greatly. |
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| Keywords: | Aegilops alloployploid genome evolution RAPD |
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