A novel endothelial tyrosine kinase cDNA homologous to platelet-derived growth factor receptor cDNA. |
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Authors: | R Sarzani G Arnaldi R De Pirro P Moretti S Schiaffino A Rappelli |
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Affiliation: | Istituto di Patologia Medica, University of Ancona, Italy. |
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Abstract: | Degenerate oligonucleotide primers complementary to the highly conserved subdomains III and VIII of subclass III tyrosine kinase receptors (TKr-III) were utilized to amplify rat aortic cDNA by polymerase chain reaction. Most of the cloned DNA products were rat platelet-derived growth factor receptor beta and macrophage-colony stimulating growth factor receptor cDNAs. Screening of the clones with probes coding for the receptor-specific kinase insert domain allowed the identification of a novel putative TKr-III cDNA, which hybridized with a approximately 6.1 kb mRNA with a distinctive tissue distribution. In situ hybridization on rat tissues and Northern analysis of cultured cells indicate that endothelial cells express a novel putative TKr-III mRNA. |
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