CO2和O3浓度倍增及其交互作用对大豆叶绿体超微结构的影响

应用透射电镜观察了模拟大气CO2和O3浓度倍增及其交互作用(开顶箱法)对大豆叶肉细胞叶绿体超微结构的影响。结果表明，CO2浓度倍增促进了大豆叶绿体的发育，内含淀粉粒积累明显增多、体积增大；叶绿体被膜保持完好；叶绿体基粒片层排列整齐，而O3浓度倍增抑制了叶绿体内淀粉粒的累积，并导致叶绿体被膜破碎，片层解体，严重地破坏了叶绿体的结构和功能CO2和O3浓度倍增的交互作用对叶绿体超微结构有不同程度的破坏，但二者浓度呈梯度增加对叶绿体的损害作用要大于二者浓度持续倍增对叶绿体的影响，进一步表明CO2正效应对O3负效应的补偿作用。

Effect of doubled CO2 and O3 concentration and their interactions on ultrastructure of soybean chloroplast

The effect of doubled CO 2 and O3 concentration on the ultrastructure of chloroplast in leaf cells of soybean was tested by open top chamber method,and examined under transmission electron microscope. The results showed that under doubled CO 2 concentration,the amount and volume of starch grains in chloroplast increased,but the chloroplast membrane and the lamellar structure still kept integrated. The accumulation of starch grains was restrained,and the chloroplast membrane and the lamellar structure were disorganized under doubled O 3 concentration. The ultrastructure of chloroplast was destroyed in different degrees under the interaction of doubled CO 2 and O 3 concentration. The damage to chloroplast arose from the abrupt doubling of CO 2 and O 3 was more severe than that from gradual increase of CO 2 and O 3 concentration. The negative effect of O 3 was partly compensated by the positive effect of doubled CO 2.