Hepatoprotective phytocompounds from Cryptomeria japonica are potent modulators of inflammatory mediators |
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Authors: | Shyur Lie-Fen Huang Chi-Chang Lo Chiu-Ping Chiu Chih-Yang Chen Yi-Ping Wang Sheng-Yang Chang Shang-Tzen |
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Institution: | Agricultural Biotechnology Research Center, Academia Sinica, Taipei 115, Taiwan, ROC. lfshyur@ccvax.sinica.edu.tw |
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Abstract: | Cryptomeria japonica is an important plantation conifer tree in Asia. This study aimed to characterize the anti-inflammatory and hepatoprotective activities of the phytocompounds from C. japonica wood on LPS- or TPA-induced activation of proinflammatory mediators and CCl(4)-induced acute liver injury in mice. A CJH7-2 fraction was purified from C. japonica extracts following bioactivity-guided fractionation, and it exhibited significant activities on inhibition of NO production and iNOS expression as well as up-regulating HO-1 expression in LPS-stimulated macrophages. CJH7-2 also potently inhibits COX-2 enzymatic activity (IC(50)=5 microg/mL) and TPA-induced COX-2 protein expression in mouse skin (1mg/200 microL/site). CJH7-2 (10 mg/kg BW) can prevent CCl(4)-induced liver injury and aminotransferases activities in mice. Chemical fingerprinting analysis showed that terpenes are the major bioactive compounds in the CJH7-2 fraction. This is the first study to demonstrate that chemical constituents from the wood extract of C. japonica possess anti-inflammatory activities in vitro and in vivo that may play a role in hepatoprotection. |
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Keywords: | Cryptomeria japonica Taxodiaceae Hepatoprotection Cycloxygenase-2 Nitric oxide Inducible nitric oxide synthase Heme oxygenase-1 Terpenoids |
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